Procedures are described for extracting glucuronic acid and sulfuric acid conjugates from animal urine by using a nonionic type of adsorption chromatography. A chromatographic column (Porapak Q coated with trioctylamine) was developed to take advantage of nonspecific adsorption characteristics of these compounds on anion-exchange columns. The separation procedure provided sufficient cleanup of the conjugates to allow for their chromatography by other chromatographic methods.