AbstractCorpora allata are the defined site of juvenile hormone (JH) biosynthesis in reproductive adult insects and their activity is regulated by intrinsic and extrinsic factors. In the adult lady beetleCoccinella septempunctata, it has been well established that the development of ovaries is controlled by JH (Guan 1987). The present study aims at the elucidation of the influence of some factors regulating reproduction of the lady beetle. They include topical application of JH analogues, presence of neuropeptides in the extract from the brain, as well as the development of ovary and type of food on CA activity. JH synthesis in CA is monitored with radiochemical assay and immunoelectrophoresis.