Four groups of Lohanas, belonging to the Gujarati, Sindhi and Punjabi were studied for various genetic markers. Lohanas have higher B than A and low Rh(D) negative (1.65–4.64%). The Hp1 gene ranges from 0.1557 to 0.2639; Gm1 is lower (0.34–0.55) than in other populations in Southern India. G-6-PD deficiency was prevalent in 3–8%. All the four groups have a high incidence of the thalassaemia trait and possess Hb-D. Hb, J, and L were also observed in two groups. Data was analysed for intergroup differ