Purification of a Flavonoid Sample by Countercurrent Chromatography
作者:
Alain Berthod,
Karine Talabardon,
François De La Poype,
ClemensA. J. Erdelmeier,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1998)
卷期:
Volume 21,
issue 19
页码: 3003-3019
ISSN:1082-6076
年代: 1998
DOI:10.1080/10826079808006882
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A quercitrin rich vegetal flavonoid sample was fractionated using a new hydrodynamic CCC apparatus based on the Ito scheme IV design. The Kromaton CCC apparatus has two spools with a tube coiling allowing work with two different apparatus volumes. The small “analytical” volume was 94 mL and the large “preparative” volume was 1.07 L. The fractionation of the flavonoid sample was done using the two machine volumes. The bi phasic liquid system was a non chlorinated solvent system, butanone/water. UV detection was possible at 330 nm. In one run, 330 mg of the flavonoid sample could be fractionated by the CCC machine in six peaks that were collected in nine fractions.
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