TEM AND SEM FINDINGS IN CAT FIBROBLASTS CULTIVATEDIN VITROWITH AND WITHOUT MYCOPLASMA
作者:
ERIK LARSSON,
ULF T. BRUNK,
期刊:
Acta Pathologica Microbiologica Scandinavica Section A Pathology
(WILEY Available online 1981)
卷期:
Volume 89A,
issue 1‐6
页码: 9-15
ISSN:0365-4184
年代: 1981
DOI:10.1111/j.1699-0463.1981.tb00180.x
出版商: Blackwell Publishing Ltd
关键词: Fibroblasts;mycoplasma contamination;TEM and SEM
数据来源: WILEY
摘要:
Mycoplasma contamination of cultured cells may cause considerable interference with their behaviour and metabolism. Whether or not mycoplasma are actually taken up by cells in vitro has long been a matter of dispute. In the present study mycoplasma were shown to be phagocytosed by feline lung fibroblasts and to end up in the lysosomal vacuome of the cells, where the organisms were only partially degraded by the hydrolytic enzymes leading to a rapid (within a few days) accumulation of secondary lysosomes of the residual body variety. It is thus obvious that studies on the structure and function of the lysosomal vacuome in cultured cells are possible only if precautions are taken to avoid that the cultures become contaminated by mycoplasma.
点击下载:
PDF
(2500KB)
返 回