Characterization of a Murine IgG3 Monoclonal Antibody againstChlamydia‐Specific Lipopolysaccharide and its Purification by Affinity Chromatography on IgG Fc‐Receptors fromStreptococcus Dysgalactiae
作者:
D. Thiele,
C. Lämmler,
R. Sting,
H. Krauss,
期刊:
Journal of Veterinary Medicine, Series B
(WILEY Available online 1991)
卷期:
Volume 38,
issue 1‐10
页码: 11-16
ISSN:0931-1793
年代: 1991
DOI:10.1111/j.1439-0450.1991.tb00839.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SummaryPurification of a murine IgG3 monoclonal antibody (Mab 22) directed against an epitope ofChlamydia‐specific lipopolysaccharide by affinity chromatography on recently described solid phase IgG Fc‐receptors fromStreptococcus dysgalactiaeis reported. SDS‐PAGE studies revealed the purity of the eluted antibody. The purified Mab 22 was characterized by determination of class, subclass and light chain‐type, and by dot tests and immunoblot a
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