AbstractWe report here the sequence of a 33,117 bp DNA fragment located approximately 30 kb from the centromere on the right arm ofSaccharomyces cerevisiaechromosome II. We have detected 16 open reading frames (ORFs) longer than 450 bp, provisionally called YBR0301 to YBR0322, covering 70·4% of the entire sequence. The ORFs YBR0301, YBR0302, YBR0303, YBR0305 and YBR0315 correspond to previously sequencedS. cerevisiaegenesGAL10, GAL1, FUR4, CAL1andL2B, respectively. Translation products of two other ORFs, YBR0308 and YBR0312 exhibit similarity to previously knownS. cerevisiaeproteins: the mitochondrially associated proteinSCO1and the protein kinase YKR2. The predicted protein product of the ORF YBR0321 shows a 41·6% identity score with theEscherichia colipyroxamine 5′‐phosphate oxidase. The nine other ORFs show no significant homology to known proteins. The sequence has been deposited in the EMBL data library under Accession Number X