Retention Behavior of Neutral Molecules in Ion Interaction Chromatography
作者:
T. Cecchi,
F. Pucciarelli,
P. Passamonti,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1998)
卷期:
Volume 21,
issue 16
页码: 2423-2433
ISSN:1082-6076
年代: 1998
DOI:10.1080/10826079808003588
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The retention behaviour of aprotic neutral molecules in Ion Interaction Chromatography was investigated. The study demonstrates that their retention, for an aqueous mobile phase (phosphate buffer pH 7.2-methanol, 85:15 v/v) containing the ion-interaction reagent (IIR) in the range 0–25 mM, decreases with increasing IIR concentration. Silanophilic interactions were accounted for by comparison of results obtained with either a silica based or a styrene-divinylbenzene based C18stationary phase, under otherwise identical conditions. The retention behaviour of neutral molecules was elucidated on the basis of the exhaustive Ion Interaction retention model and evidence was obtained to support the proposition that the decreased retention is the result of an adsorption competition between tested analytes and ion-interaction reagent for inner layer sites on the stationary phase.
点击下载:
PDF (495KB)
返 回