Rapid and Sensitive Determination of Meropenem in Rat Plasma by High Performance Liquid Chromatography
作者:
A. Dupuis,
Ph. Minet,
W. Couet,
Ph. Courtois,
S. Bouquet,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1998)
卷期:
Volume 21,
issue 16
页码: 2549-2560
ISSN:1082-6076
年代: 1998
DOI:10.1080/10826079808003598
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A sensitive and rapid high-performance liquid chromato-graphic method using UV detection, has been developed for the analysis of meropenem in plasma. This involved a single liquid-solid extraction on Isolute C18-EC column in the presence of an internal standard (ceftazidime). Analysis was performed by isocratic elution with a mobile phase consisting of 0.01 M acetate buffer pH 4.8 containing 15% (v/v) methanol, with UV detection at 296 nm. The limit of quantitation of the assay was 0.125 mg/L. The method was applied to a pharmacokinetic study in rats receiving 100 mg/kg by IV route with a good accuracy (94–101%) and precision (less than 13%).
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