The Mexican and Central American species ofAmblyopinusSolsky 1875, adults of which are usually found in the fur of rodents, are revised. All species are fully described, and keys and illustrations of diagnostic features are provided to aid in their identification. A substantial quantity of new host and distributional data is provided. Five species, one of which consists of two subspecies, are known from Mexico and Central AmericaAmblyopinus emarginatusSeevers 1955(sanbornigroup) occurs in the montane regions of northwestern Panama and Costa Rica where it occurs onOryzomys albigularis.In addition, four species and two subspecies of thejelskiigroup occur in Central America and Mexico:A. isabellaeBarrera 1966, Sierra Madre del Sur in Mexico (host[s] various species ofPeromyscusandNeotoma); A. barreraiZaragoza Caballero & Sánchez Hernández 1993, Sierra Madre de Oaxaca in Mexico (host[s],Peromyscusspecies, especiallyP. melanocarpus); A. schmidti schmidtiSeevers 1944, highlands of Chiapas and Guatemala (host, primarilyPeromyscus guatemalensis); A. schmidti bolivariBarrera et al. 1960, Transvolcanic Sierra of Mexico (host, primarilyMicrotus mexicanus); A. titoniBarrera 1966, highlands of northwestern Panama and Costa Rica (host[s],Peromyscus nudipesandReithrodontomys creper).The hosts ofA. emarginatusin both Central and South America are complex-penis cricetines, primarilyOryzomys albigularis.However, the Mexican and Central American members of thejelskiigroup exhibit two major host transfers: to simple-penis peromyscine rodents(A. isabellae, A. barrerai, A. s. schmidti, A. tipitoni);and, to microtine rodents of the genusMicrotus (A. s. bolivari). All five taxa of thejelskiigroup have allopatric ranges in different mountain systems. OnlyA. tiptoniandA. emarginatushave sympatric ranges in the montane regions of northwestern Panama and Costa Rica, but these two species are in different species groups and live on different hosts.