An enhanced chemiluminescent enzyme immunoassay for serum carcinoembryonic antigen based on a modification of a commercial kit
作者:
R. Haggart,
G. H. G. Thorpe,
S. B. Moseley,
L. J. Kricka,
T. P. Whitehead,
期刊:
Journal of Bioluminescence and Chemiluminescence
(WILEY Available online 1986)
卷期:
Volume 1,
issue 1
页码: 29-34
ISSN:0884-3996
年代: 1986
DOI:10.1002/bio.1170010106
出版商: John Wiley&Sons, Ltd.
关键词: Enhanced chemiluminescence;enzyme immunoassay;carcinoembryonic antigen
数据来源: WILEY
摘要:
AbstractA conventional colorimetric peroxidase end‐point (ortho‐phenylenediamine substrate), used in an enzyme immunoassay for carcinoembryonic antigen, employing plastic beads as solid support, has been replaced by a much faster (30 seconds versus 30 minutes) enhanced chemiluminescent assay for the peroxidase label.Para‐iodophenol was used to enhance the light emission from the peroxidase catalysed chemiluminescent reaction between luminol and hydrogen peroxide. Values for precision and carcinoembryonic antigen concentration obtained with the chemiluminescent and colorimetric versions of the immunoassay on 62 serum specimens were in good agre
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