首页   按字顺浏览 期刊浏览 卷期浏览 An enhanced chemiluminescent enzyme immunoassay for serum carcinoembryonic antigen base...
An enhanced chemiluminescent enzyme immunoassay for serum carcinoembryonic antigen based on a modification of a commercial kit

 

作者: R. Haggart,   G. H. G. Thorpe,   S. B. Moseley,   L. J. Kricka,   T. P. Whitehead,  

 

期刊: Journal of Bioluminescence and Chemiluminescence  (WILEY Available online 1986)
卷期: Volume 1, issue 1  

页码: 29-34

 

ISSN:0884-3996

 

年代: 1986

 

DOI:10.1002/bio.1170010106

 

出版商: John Wiley&Sons, Ltd.

 

关键词: Enhanced chemiluminescence;enzyme immunoassay;carcinoembryonic antigen

 

数据来源: WILEY

 

摘要:

AbstractA conventional colorimetric peroxidase end‐point (ortho‐phenylenediamine substrate), used in an enzyme immunoassay for carcinoembryonic antigen, employing plastic beads as solid support, has been replaced by a much faster (30 seconds versus 30 minutes) enhanced chemiluminescent assay for the peroxidase label.Para‐iodophenol was used to enhance the light emission from the peroxidase catalysed chemiluminescent reaction between luminol and hydrogen peroxide. Values for precision and carcinoembryonic antigen concentration obtained with the chemiluminescent and colorimetric versions of the immunoassay on 62 serum specimens were in good agre

 

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