AbstractResponse of sunflower (Helianthus annuusL.) to sulphur applied ca. 0, 30, 90 and 150 mg kg‐1and nitrogen applied ca. 0, 100 and 200 mg kg‐1soil were studied on a Typic Camborthids in greenhouse. Nitrogen and S application had a significant (P<0.01) main and interactive effect on seed yield and dry weight of leaves and stem. Cumulative N and S contents of sunflower seeds, stem and leaves were significantly (P<0.01) affected by N and S application. Sulphur transport and accumulation in sunflower seeds was increased with S application. There were significant (P<0.01) correlations between seed yield and N and S content of sunflower plants. Critical S concentration (%) in 60 days old plants was 0.36 for 95 % relative yield of the maximum. Application of 90 mg S kg‐1with 200 mg N kg‐1resulted in the highest seed yield and maximum total S and N contents in sunflower leaves, stem and seeds. Relative increase in sunflower seeds yield at higher levels of N and S was more than that of dry weight of stem and