This paper presents the results of NAA analysis of 43 samples of archaeological glass (Bronze Age to Roman period) found in Bohemia (with the exception of one comparative sample of Mediterranean origin). A detailed description of the method used is given. The applicability of this type of analysis to prehistoric glass from the technical point of view and the validity of its results for historical studies is tested. In general, the method succeeded in distinguishing major clusters of glass. as recognized by means of prehistoric studies, though without showing more intensively the expected differences within these clusters.