Functional Assay of a PutativeDrosophilaSodium Channel Gene in Homozygous Deficiency Neurons
作者:
GermeraadSusan,
O'dowdDiane,
AldrichRichard W.,
期刊:
Journal of Neurogenetics
(Taylor Available online 1992)
卷期:
Volume 8,
issue 1
页码: 1-16
ISSN:0167-7063
年代: 1992
DOI:10.3109/01677069209167268
出版商: Taylor&Francis
关键词: voltage-clamp;β-galactosidase;heat shock promoter
数据来源: Taylor
摘要:
Using voltage-clamp techniques, we have examined embryonic sodium currents in neurons deficient for a gene located at 60E5/6 that shares extensive amino acid similarity with vertebrate sodium channel genes. These neurons expressed sodium currents similar to wildtype, supporting the hypothesis thatpara, and not the gene at 60E5/6, is the primary sodium channel gene expressed in embryonic neurons. A simple marking procedure allowing positive identification of the genotypes of culturedDrosophilaembryos obtained from heterozygous parents was used to recognize cultures homozygous for deficiencies. The morphological development of both neurons and myotubes in these cultures was similar to wildtype, making it feasible to compare the properties of normal diploid cells and cells completely lacking a putative sodium channel gene.
点击下载:
PDF (1353KB)
返 回