The Effect of Diamide (Azodicarboxylic Acid-bis-Dimethylamide) on Sulfhydryl Group Content, Proteins, and the Location of Phosphatidylethanolamine in Human Blood Platelets
作者:
G. Ostermann,
P. Spangenberg,
M. Meyer,
F.H. Herrmann,
U. Till,
期刊:
Acta Haematologica
(Karger Available online 1982)
卷期:
Volume 68,
issue 4
页码: 278-284
ISSN:0001-5792
年代: 1982
DOI:10.1159/000206993
出版商: S. Karger AG
关键词: Diamide;Human blood platelets;Phosphatidylethanolamine labeling;SDS electrophoresis;Sulfhydryl groups
数据来源: Karger
摘要:
Treatment of human blood platelets with the thiol-oxidizing agent, diamide, causes rapid oxidation of glutathione and alterations in aggregation and release reaction. Moreover, cross-linking of proteins was observed. Three high molecular weight bands of 200 – 240 × 103 daltons and a band of 66 × 103 daltons were involved in this process. After reduction with dithiotreitol the normal pattern was received. In contrast to the erythrocyte, the cross-linking of platelet proteins was not accompanied by a reorientation of phosphatidylethanolamine in the membrane. Also a considerably smaller effect of diamide on platelet protein sulfhydryl group content was measu
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