Variability of Fiber Size, Capillary Density and Capillary Length Related to Horse Muscle Fixation Procedures
作者:
Laurence Mermod,
Hans Hoppeler,
Susan R. Kayar,
Reto Straub,
Ewald R. Weibel,
期刊:
Cells Tissues Organs
(Karger Available online 1988)
卷期:
Volume 133,
issue 2
页码: 89-95
ISSN:1422-6405
年代: 1988
DOI:10.1159/000146621
出版商: S. Karger AG
关键词: Capillary;Electron microscopy;Muscle fiber;Morphometry-Stereology;Horse;Fixation technique;Anisotropy
数据来源: Karger
摘要:
Muscle samples were taken from the superficial part of the semitendinosus muscle of four standardbred horses to compare the effects of three different fixation procedures on fiber size and capillarity. Infiltration fixation in an extended position of the muscle was compared to immersion fixation and infiltration fixation in a flexed position of the limb. The capillary length density was used to estimate muscle capillarity. Infiltration fixation was found to be a viable alternative to perfusion fixation of muscles for morphometry of fiber size and capillary length density allowing muscle tissue fixation at a defined sarcomere length without fiber kinking.
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