A modified polyamidoamine-starburst dendrimer (PAMAM-SBD), 16-cascade:(1,4-diaminobutane)[4-1,4]:(1,6-diaza-7-oxo-nonyl idene)2:propanoic acid methyl ester (DABD), was synthesized and used as a carrier in electrokinetic capillary chromatography. The utility of DABD is demonstrated with the separation of a mixture of naphthalene derivatives and five neutral aromatic molecules without the need for an organic solvent as a modifier. Separation of a mixture of five neutral aromatic molecules were optimized through dendrimer concentration, pH, and organic modifier. The modified polyamidoamine-starburst dendrimer offered selectivity for polynuclear aromatic hydrocarbons as well as naphthalene based derivatives. However, DABD did not provide as much selectivity for neutral aromatic compounds in the presence of organic modifier.