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High Pressure Liquid Chromatographic Determination of Hydrochlorothiazide (HCT) in Pharmaceutical Preparations and Human Serum After Solid Phase Extraction

 

作者: I.N. Papadoyannis,   V.F. Samanidou,   K.A. Georga,   E. Georgarakis,  

 

期刊: Journal of Liquid Chromatography & Related Technologies  （Taylor Available online 1998）
卷期: Volume 21, issue 11  

页码: 1671-1683

 

ISSN:1082-6076

 

年代: 1998

 

DOI:10.1080/10826079808001251

 

出版商: Taylor & Francis Group

 

数据来源: Taylor

 

摘要:

A rapid, accurate, and sensitive method has been developed for the quantitative determination of hydrochlorothiazide (HCT), an antihypertensive-diuretic agent. A Nucleosil C18100×4.6 mm, 5 μm analytical column was used with a mixture of CH3CN-1% acetic acid, at a volume ratio 20:80. Detection was performed with a variable wavelength UV-visible detector at 270 nm, resulting in detection limits of 0.2 ng per 20 μL injection.

 

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