The carbohydrate‐containing Traction ofCandida albicanswas isolated from the crude extract with ConA Sepharose affinity chromatography and studied by IgE‐immuno‐blotting with individual and pooled sera fromC. albicans‐allergic subjects. In the ConA‐bound fraction there was a diffuse IgE binding in the high molecular weight area which also gave a carbohydrate stain (PAS). A distinct band corresponding to a molecular weight of 70 kD bound specific IgE antibodies. This glycoprotein, presumably a mannoprotein, gave a weak carbohydrate staining and a strong protein staining. further biochemical studies are needed to reveal the exact nature of the epitopes in the ConA‐bound mannose‐containing fraction