AbstractRaman excitation profiles (REPs) for bands of carotenoid and chlorophyll b (Chl b) from whole cells of green algae at low temperatures are presented. In addition to preventing thermal destruction of the samples during long‐term irradiation, the use of a double channel surface scanning technique allowed the recording of the Raman spectrum of a non‐resonating substance such as acetone suitable as an intensity reference at the same time. The measured REPs are compared with the absorption spectrum of whole cells at low temperatu