Determination of Biogenic Amines In Rat Brain Dialysates by High Performance Liquid Chromatography
作者:
R. Durán,
M. Alfonso,
B. Arias,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1998)
卷期:
Volume 21,
issue 18
页码: 2799-2811
ISSN:1082-6076
年代: 1998
DOI:10.1080/10826079808003443
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A general problem found in the analysis of samples from brain microdialysis is the existence of some substances which can interfere with the dopamine. In our chromatographic conditions, we found an interfering peak with the following mobile phases: acetic acid / sodium acetate 1M (with 5% methanol and 1mM EDTA), and citric acid / sodium phosphate (with 5% methanol and 2mM EDTA). The solution for this problem was to employ a mobile phase consisting of KH2PO4(with lmM EDTA and 5% methanol), with an ion-pair agent of sodium salt of 1-octanesulfonic acid. The interfering peak was identified as the uric acid (resulting from the cellular metabolism) based on its chromatographic properties.
点击下载:
PDF (471KB)
返 回