Studies on Bovine Leukosis
作者:
A.A. Ressang,
A.L.J. Gielkens,
J. Quak,
Miss Nel Mastenbroek,
期刊:
Veterinary Quarterly
(Taylor Available online 1981)
卷期:
Volume 3,
issue 1
页码: 31-33
ISSN:0165-2176
年代: 1981
DOI:10.1080/01652176.1981.9693791
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
An enzyme linked immunosorbent assay (ELISA) and the agar gel immunodiffusion test with bovine leukosis virus glycoprotein as antigen (AGIDT‐BLV gp) were further used to test 633 bovine sera for antibodies to BL V. Both tests detected the same number of sera positive (149) or negative (464) for antibodies. Nine sera were negative in the ELISA but found to be weakly positive (2 sera) or bending the control line (7) in the AGIDT‐BLV gp. On the other hand 11 sera were scored negative in the AGIDT‐BLV gp but were weakly positive (9 sera), positive (1), and strongly positive (I) in the ELISA. Both tests are used routinely in this Institute as they complement each other, specially if sera with low antibody titers are under investigation. It is concluded that ELISA can fully replace radioimmunoassays in the serodiagnosis of enzootic bovine leukosis.
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