Immunoelectron microscopic studies of immune complex deposits and basement membrane components in IgA nephropathy
作者:
David F. Woodrow,
Ian Shore,
Jill Moss,
Peter Gower,
Malcolm Phillips,
期刊:
The Journal of Pathology
(WILEY Available online 1989)
卷期:
Volume 157,
issue 1
页码: 47-57
ISSN:0022-3417
年代: 1989
DOI:10.1002/path.1711570107
出版商: John Wiley&Sons, Ltd.
关键词: Immunoelectron microscopy;immunogold;mesangial IgA nephropathy;type IV collagen;fibronectin
数据来源: WILEY
摘要:
AbstractThe relationship between the immune complex deposits of mesangial IgA nephropathy and the basement membrane components, type IV collagen and fibronectin, has been investigated by an indirect immunogold technique in four cases of mesangial IgA disease. Using paraformaldehyde‐fixed, Lowicryl K4M resin‐embedded kidney, IgA, IgM and C3 were localized in the mesangial electron‐dense deposits with 10 and 20 nm gold‐labelled secondary antibodies. In the same glomeruli, type IV collagen and fibronectin were rarely present within the electron‐dense deposits, although both were distributed throughout the remainder of the mesangial matrix with the exception of the subepithelial regions. These two components were also present within the glomerular basement membrane and localized mainly on the endothelial aspect. A similar distribution of the basement membrane components was seen in a control kidney processed in the same way.This technique gives reproducible results and has demonstrated for the first time the relationship between the mesangial immune complex deposits of mesangial IgA nephropathy and the basement membrane components of the matrix in which they
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