High-Sensitivity Antitumor Drug Sensitivity Testing
作者:
Tatsuhei Kondo,
Kimio Wada,
Miyako Kawashima,
Yoshitaka Sato,
Masaji Yamauchi,
期刊:
Oncology
(Karger Available online 1994)
卷期:
Volume 51,
issue 6
页码: 535-539
ISSN:0030-2414
年代: 1994
DOI:10.1159/000227399
出版商: S. Karger AG
关键词: Antitumor drug sensitivity testing;Succinic dehydrogenase inhibition test;XTT assay
数据来源: Karger
摘要:
For antitumor drug sensitivity testing we have been performing the succinic dehydrogenase inhibition test using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide (MTT). A new tetrazolium salt 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl-amino) carbonyl]-2H-tetrazolium hydroxide (XTT) has been synthesized recently, and we have been conducting basic studies using it. We were able to obtain a high degree of sensitivity by adding phenazine methosulfate as a promoter in this assay. In these assays, there was a positive correlation between absorbance and cell count and XTT assay was more sensitive than MTT assay. In XTT assay, the production of formazan increases with reaction time over a protracted period of time, we assessed the possibility of performing assays with fewer cells than MTT was used. The results using cancer cell lines showed that when reacted for a longer time (6 h), it was possible to perform adequate assays using this method with 5,000 cells per well, and it will be useful when the amount of biopsy specimen is limited. We also evaluated the inhibition index of each of the drugs, comparing it with the MTT assay.
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