Einige Probleme bei der Anwendung des MPN‐Verfahrens zur quantitativen Salmonellenbestimmung
作者:
M. Nagel,
W. Stelzer,
E. Schulze,
期刊:
Acta hydrochimica et hydrobiologica
(WILEY Available online 1983)
卷期:
Volume 11,
issue 1
页码: 23-35
ISSN:0323-4320
年代: 1983
DOI:10.1002/aheh.19830110107
出版商: WILEY‐VCH Verlag GmbH
数据来源: WILEY
摘要:
So far, the MPN technique has been the method chosen for detecting pathogenic bacteria in waters, since it allows us to use larger volumes. In the evaluation, however, comparatively frequently (10 … 15%) nontypical codes occur. The validity of the Poisson model is confirmed for pure cultures by extensive laboratory experiments with pure cultures and controlled mixed cultures. In mixed cultures, however, a comparatively large number of atypical codes occur, since obviously a single organism is not sufficient for the formation of a growth‐cover on the test tube, so that the true number of germs may be considerably underestimated. Obviously, the accompanying flora has an inhibiting effect on the salmonella detect
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