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Einige Probleme bei der Anwendung des MPN‐Verfahrens zur quantitativen Salmonellenbestimmung

 

作者: M. Nagel,   W. Stelzer,   E. Schulze,  

 

期刊: Acta hydrochimica et hydrobiologica  (WILEY Available online 1983)
卷期: Volume 11, issue 1  

页码: 23-35

 

ISSN:0323-4320

 

年代: 1983

 

DOI:10.1002/aheh.19830110107

 

出版商: WILEY‐VCH Verlag GmbH

 

数据来源: WILEY

 

摘要:

So far, the MPN technique has been the method chosen for detecting pathogenic bacteria in waters, since it allows us to use larger volumes. In the evaluation, however, comparatively frequently (10 … 15%) nontypical codes occur. The validity of the Poisson model is confirmed for pure cultures by extensive laboratory experiments with pure cultures and controlled mixed cultures. In mixed cultures, however, a comparatively large number of atypical codes occur, since obviously a single organism is not sufficient for the formation of a growth‐cover on the test tube, so that the true number of germs may be considerably underestimated. Obviously, the accompanying flora has an inhibiting effect on the salmonella detect

 

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