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Replication and Serial Passage of a Singly Enveloped Baculovirus ofOrgyia leucostigmain Homologous Cell Lines

 

作者: S.S. Sohi,   Jean Percy,   B.M. Arif,   J.C. Cunningham,  

 

期刊: Intervirology  （Karger Available online 1984）
卷期: Volume 21, issue 1  

页码: 50-60

 

ISSN:0300-5526

 

年代: 1984

 

DOI:10.1159/000149502

 

出版商: S. Karger AG

 

关键词: Orgyia leucostigma;White-marked tussock moth;Nuclear polyhedrosis virus;Baculovirus;Cell lines;Cell cultures;In vitro replication

 

数据来源: Karger

 

摘要:

A singly enveloped nuclear polyhedrosis virus (SNPV) of the white-marked tussock moth, Orgyia leucostigma SNPV was serially passaged 60 times each in OL-12 and OL-13 cells. Percentage infected cells and PIB production stayed generally high throughout serial passaging in OL-13 cells, but declined sharply after the 41st passage in OL-12 cells. PIBs from the 4th passage of the virus in OL-12 cells were tested and found to be pathogenic to O. leucostigma larva

 

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