Immunocytochemical Methods in Cell Cultures for Evaluation of Cell Cycle and Differentiation in Normal and Neoplastic Tissues
作者:
MartinCarlos G.,
RegaderaJavier,
HernandezArturo,
CoboPaz,
NistalManuel,
期刊:
Journal of Histotechnology
(Taylor Available online 1996)
卷期:
Volume 19,
issue 2
页码: 109-114
ISSN:0147-8885
年代: 1996
DOI:10.1179/his.1996.19.2.109
出版商: Taylor&Francis
关键词: cell cultures;cell differentiation;cell proliferation;immunohistochemistry
数据来源: Taylor
摘要:
AbstractPrimary cell culture, tissue culture, and established cell line culture methods are addressed in this paper. These methods are easily applicable to the pathology laboratory. Sterile conditions to obtain, process, disaggregate the surgical specimen, and incubate the cells are emphasized. Passage to multiwell-antisera saving plates, specific streptavidin-biotin-peroxidase and phosphatase-antiphosphatase method modifications, and bromodeoxyurydine incorporation in cell culture medium are described to evaluate the cellular proliferation. We also discuss new features about conservation of easily-preserved slides that can be filed as biopsies. Satisfactory results have been obtained using immunohistochemical methods in cell cultures with antibodies against vimentin, glial fibrillary acidic protein, muscle actin, proliferating cell nuclear antigen, bromodeoxyurydine, and AE1 keratin. (The J Histotechnol19:109, 1996)
点击下载:
PDF (1049KB)
返 回