Immunohistochemical Study on the Endocrine Pancreas of Cattle with Special Reference to Coexistence of Serotonin and Glucagon or Bovine Pancreatic Polypeptide
作者:
Seigo Nakajima,
Nobuo Kitamura,
Junzo Yamada,
Tadayuki Yamashita,
Tohru Watanabe,
期刊:
Cells Tissues Organs
(Karger Available online 1988)
卷期:
Volume 131,
issue 3
页码: 235-240
ISSN:1422-6405
年代: 1988
DOI:10.1159/000146522
出版商: S. Karger AG
关键词: Endocrine pancreas;Immunohistochemistry;Serotonin Cattle;Light microscopy
数据来源: Karger
摘要:
Bovine pancreatic endocrine cells were investigated by light microscopic immunohistochemistry. Serotonin-immunoreactive cells as well as insulin-, glucagon-, somatostatin-, bovine pancreatic polypeptide (BPP)-immunoreactive cells were detected in the pancreatic islets. Generally, insulin-immunoreactive cells were distributed throughout the islet and the others took peripheral location. Since the distribution and shape of serotonin-immunoreactive cells were very similar to glucagon- and BPP-immunoreactive cells, serial sections were restained by using the elution method. All glucagon- and BPP-immunoreactive cells also showed serotonin immunore activity but glucagon and BPP immunoreactivities were never observed to be colocalized in the same cell. A small number of serotonin-immunoreactive cells were observed that showed serotonin immunoreactivity only.
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