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Simple flow microphotometer for rapid cell population analysis

 

作者: W. G. Eisert,   R. Ostertag,   E.‐G. Niemann,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1975)
卷期: Volume 46, issue 8  

页码: 1021-1024

 

ISSN:0034-6748

 

年代: 1975

 

DOI:10.1063/1.1134399

 

出版商: AIP

 

数据来源: AIP

 

摘要:

A simple particle analyzer has been developed using a modified high speed flow‐through technique and absorption measurements of a continuous He–Ne laser beam. From pulse‐shape analysis and the integration of absorption pulses, size distributions of stained or unstained cells can be derived. The range of 5–300 &mgr; is covered without changing the flow system and a rate up to 200 000/sec can be achieved. Typical applications in cell analyses are shown.

 

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