Simple flow microphotometer for rapid cell population analysis
作者:
W. G. Eisert,
R. Ostertag,
E.‐G. Niemann,
期刊:
Review of Scientific Instruments
(AIP Available online 1975)
卷期:
Volume 46,
issue 8
页码: 1021-1024
ISSN:0034-6748
年代: 1975
DOI:10.1063/1.1134399
出版商: AIP
数据来源: AIP
摘要:
A simple particle analyzer has been developed using a modified high speed flow‐through technique and absorption measurements of a continuous He–Ne laser beam. From pulse‐shape analysis and the integration of absorption pulses, size distributions of stained or unstained cells can be derived. The range of 5–300 &mgr; is covered without changing the flow system and a rate up to 200 000/sec can be achieved. Typical applications in cell analyses are shown.
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