Histological repair of the cervical epithelium after CO2 laser conization was studied using resected uterus specimens. (1) Squamous epithelium (ectocervical side) and reserve cells (endocervical side), were seen immediately after conization; (2) HE staining showed that the S-C junction was formed within 4 weeks. (3) Endocervical epithelial repair of the S–C junction tended to be delayed, and the new S–C junction was deeper in the endocervical canal than before conization; (4) The stroma was repaired within 7 weeks, by prompt collagenization.