The potential for the application of chromatography to the analysis of fatty acids was first realized by A. J. James and A. J. P. Martin in 19521. These two noted scientists successfully separated the iso- and ante-isomers of short chain free fatty acids by gas liquid chromatography (GLC). Even today, 35 years later, the method of choice for characterization of fatty acids is capillary gas chromatography with a mass spectrometer as a detector. However, high performance liquid chromatography (HPLC) is now becoming competitive in the separation of fatty acids, especially on the preparative scale.