Quantitative Endotoxin Determination in Blood – Chromogenic Modification of the Limulus Amebocyte Lysate Test
作者:
D. Berger,
E. Marzinzig,
M. Marzinzig,
H.G. Beger,
期刊:
European Surgical Research
(Karger Available online 1988)
卷期:
Volume 20,
issue 2
页码: 128-136
ISSN:0014-312X
年代: 1988
DOI:10.1159/000128751
出版商: S. Karger AG
关键词: Endotoxemia;Quantification;Limulus amebocyte lysate;Chromogenic test
数据来源: Karger
摘要:
A newly developed modification of the limulus amebocyte lysate test for quantification of endotoxin levels in blood is described. The chromogenic peptide carbobenzoxy-Gly-Gly-Arg-4-methyl-cumarinyl-7-arnid proved to be most suitable. The liberated fluorescent dye is diazotized with N(1-naphtyl-)-ethylen-diamin-dihydrochloride. Using this statistically proved reliable and sensitive test, endotoxin serum levels of healthy persons and patients undergoing major surgical treatment were compared. In the postoperative phase endotoxin serum levels up to 0.5 ng/ml can be detected without clinical signs of septicemia. Healthy persons show endotoxin serum levels up to 0.08 ng/ml. In rats no difference of endotoxin serum levels was detected in the portal vein, and in arterial and venous blood. So a physiological endotoxin resorption from the intestine followed by a clearance during the liver passage seems to be doubtful in this species.
点击下载:
PDF
(1124KB)
返 回