AbstractThe complete DNA sequence of cosmid clone pEKG080 comprising 20 723 base pairs was determined from an ordered set of subclones. The sequence contains nine open reading frames (ORFs) longer than 100 amino acids. The deduced amino acid sequence of YKL067 exhibits significant similarity to nucleoside diphosphate kinases from a number of species and probably represents this gene in yeast. YKL068 is identical to theNUP100gene recently cloned and sequenced. The gene codes for a nuclear pore complex protein. YKL073 is identical to Hsp70 proteins from yeast andHydraat about 20% of the amino acids. The significance of this similarity is uncertain. The remaining six ORFs do not share homology with known proteins. Three tRNAs for histidine, valine and tryptophan respectively were identified, the latter interrupted by a 34 bp intron. Solo delta sequences were found at three locations. The nucleotide sequence data have been deposited in the EMBL and GenBank data libraries under Accession Number X75780.