A Biamperohetric Method for the Determination of Nad-Dependent Reactions Using Tubular Carbon Electrodes in Flowing Streams
作者:
Abdulrahman S. Attiyat,
E. Lawrence Gulberg,
Gary D. Christian,
期刊:
Instrumentation Science & Technology
(Taylor Available online 1979)
卷期:
Volume 9,
issue 3
页码: 261-275
ISSN:1073-9149
年代: 1979
DOI:10.1080/10739147908543471
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Open tubular carbon electrodes were used for biamperometric measurements of iron (II) in the ferrocyanide/ferricyanide redox couple. This measurement was used to monitor dehydrogenase enzyme reactions by reaction of ferricyanide with reduced nicotinamide-adeninedinucleotide (NADH) in the presence of the enzyme diaphorase. Reagents and samples are mixed in a flowing stream and the electrodes are placed downstream to measure the extent of reaction after a constant time interval. Ethanol was determined by reaction with NAD in the presence of alcohol dehydrogenase (ADH), ferricyanide, and diaphorase. Optimum solution conditions for the above measurements are described.
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