Test protocol of an enzyme‐linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukosis virus
作者:
A. L. J. Gielkens,
A. A. Ressang,
J. IJzermann,
J. Quak,
期刊:
Veterinary Quarterly
(Taylor Available online 1981)
卷期:
Volume 3,
issue 1
页码: 34-37
ISSN:0165-2176
年代: 1981
DOI:10.1080/01652176.1981.9693792
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
In this communication the test procedure is described for an indirect enzyme‐linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukosis virus (BLV). Test sera are incubated in polystyrene microtiter plates sensitized with a partly‐purified preparation of BLV. Bovine antibodies are detected with anti‐species immunoglobulin conjugated to the enzyme horseradish peroxidase, followed by the addition of the enzyme substrate.
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