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Impact of Ischemia on Tissue Oxygenation and Wound Healing: Intravital Microscopic Studies on the Hairless Mouse Ear Model

 

作者: M. Kamler,   H.-A. Lehr,   J.H. Barker,   R.K. Saetzler,   T.J. Galla,   K. Messmer,  

 

期刊: European Surgical Research  (Karger Available online 1993)
卷期: Volume 25, issue 1  

页码: 30-37

 

ISSN:0014-312X

 

年代: 1993

 

DOI:10.1159/000129254

 

出版商: S. Karger AG

 

关键词: Tissue oxygenation;Buflomedil;Wound healing;Ischemia

 

数据来源: Karger

 

摘要:

To determine the effect of ischemia on tissue oxygenation and the healing of experimental wounds, chronic ischemia was induced in the ears of hairless mice by ligating 2 of the 3 main nutritional arteries. Tissue ischemia was verified by measurement of transcutaneous pO2 (tcp O2)prior to and on days 3, 6, 9,12 and 15 after vessel ligation. TcpO2values decreased from 24 to 6 mm Hg at day 2 after ligation, and slowly recovered to 12 mm Hg at day 12 after vessel ligation. In animals treated with the vasoactive drag buflomedil (3 mg/kg BW i.v., starting 2 days after vessel ligation) tcpO2values were significantly higher on days 6 and 9 when compared to saline-treated control animals. In order to find out whether the enhanced tissue oxygenation resulted in enhancement of healing of wounds, we created circular wounds (diameter = 2.5 mm, depth 0.1 mm) on ischemic ears 2 days after vessel ligation. The wound surface area was measured by means of intravital fluorescence microscopy and digital planimetry at 3-day intervals until the time of wound closure. These experiments were performed on buflomedil-treated and control animals receiving equivalent amounts of saline. The reduction of wound surface area was accelerated and wound closure time was reduced from 15 days in control animals to 12 days in buflomedil-treated animals (p < 0.01). Functional capillary density as well as the microhemodynamic parameters microvessel diameter and red blood cell velocity were not different between buflomedil-treated and control animals.

 

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