Osteopontin adhesion receptors on gingival fibroblasts
作者:
J. A. D'Errico,
J. J. Sauk,
C. W. Prince,
M. J. Somerman,
期刊:
Journal of Periodontal Research
(WILEY Available online 1995)
卷期:
Volume 30,
issue 1
页码: 34-41
ISSN:0022-3484
年代: 1995
DOI:10.1111/j.1600-0765.1995.tb01250.x
出版商: Blackwell Publishing Ltd
关键词: osteopontin;integrin;vitronectin;attachment
数据来源: WILEY
摘要:
Osteopontin (OPN) promotes attachment and spreading of cells in an RGD dependent fashion, suggesting that OPN interacts with integrins on cell surfaces. Here in, we show that LM‐609, a monoclonal antibody to the αvβ3integrin (a vitronectin receptor), inhibited OPN‐mediated attachment of gingival fibroblasts. To characterize the cell surface receptors responsible for this interaction, we performed OPN‐sepharose affinity chromatography using detergent extracts of35S‐methionine orl25I‐surface labeled gingival fibroblasts. Proteins bound to the OPN‐matrix were eluted with EDTA and subjected to SDS‐PAGE under reducing conditions. EDTA eluates from both125I‐surface labeled and35Smethionine labeled extracts demonstrated prominent bands in the 90kDa and 50kDa regions, by both autoradiography and fluorography, respectively. These studies suggest that OPN is associated with other cell surface molecules in addition to αvβ3. Furthermore, these as yet to be characterized proteins, may prove to have a stronger affinit
点击下载:
PDF
(4230KB)
返 回