The purpose of this study was to identify the accumulation of mutagens and/or promutagens in fish using the AmesSalmenella/mammalian microsome assay. The fish were taken from four different collection sites in Northeast Tennessee area: Watauga Lake, Boone Lake, the Nolichucky River, and the Holston River. A gross autopsy vas performed on each fish; this autopsy consisted of recording weight, length, age, sex, and the physical condition of the various fish organs. Each fish (whole body) was extracted using ultra‐pure Z‐propanol as the solvent.