Invertase biosynthesis in Saccharomyces cerevisiae 01K32 cells after alcohol fermentation using cane molasses has been carried out. At TRS concentration of 4%, a maximum degree of intracellular invertase production (140–150 E/g) has been reached for 6 hours. The sugars induction effect in molasse medium is registered to the 3% concentration in case of using for invertase biosynthesis the strain only. A two-stage culture of the strain examined has been carried out in a bioreactor and a four times increase of intracellular invertase activity has been reached.