The effects of humour on health were investigated using a sample of 504 individuals comprising three groups (community group, university students, and respondents with a medical condition). Hypotheses were:1that after controlling for other variables, humour would be significantly associated with health:2that individuals with a greater sense of humour would report significantly higher levels of good health as compared with those with less humour; and3that the assessment of the factor structure of the Multidimensional Sense of Humour Scale (MSHS) would support its construct validity in the Australian context.The present findings supported the view that a sense of humour is associated with health, and also provided support for the validity and reliability of the MSHS instrument.