SORPTION OFSTREPTOCOCCUS FAECIUMTO GLASS
作者:
DAG ØRSTAVIK,
期刊:
Acta Pathologica Microbiologica Scandinavica Section B Microbiology
(WILEY Available online 1977)
卷期:
Volume 85B,
issue 1
页码: 38-46
ISSN:0304-131X
年代: 1977
DOI:10.1111/j.1699-0463.1977.tb01672.x
出版商: Blackwell Publishing Ltd
关键词: Sorption;Streptococcus faecium;glass
数据来源: WILEY
摘要:
A method has been developed by which to study the sorption ofStreptococcus faeciumto soda‐lime cover glasses. Conditions were chosen to minimize the influence on sorption of bacterial polymer production, passive sorption being studied rather than attachment mediated by metabolic activities. Sorption ofS. faeciumincreased with increasing temperature (to 50d̀ C), time, and cell concentration, but equilibrium apparently was not reached even after incubation for 8 hours or at a cell concentration of 3 times 1010per ml. Sorption increased with solute molarity up to 0.1 M concentration of NaCl and KCl, indicating an effect of the electrical double layers on the apposition of cells to the glass surface. Desorption of bacteria could be obtained after multiple washings of the glasses in buffer or by the action of Tween 80, but not if sorbed bacteria were left in distilled water, various salt solutions, urea, or in suspensions of unlabelled bacteria. It was concluded that sorption occurred as a result of chemical interactions between the glass and the cell surface. Tween 80 at a concentration of 1 per cent inhibited sorption to 26 per cent of buffer controls, 2 M urea was less effective, and 1 M NaCl was without effect. It is suggested that hydrophobic interactions may be of importance in the binding ofS. faeciumto gla
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