Confocal differential interference contrast (DIC) microscopy: including a theoretical analysis of conventional and confocal DIC imaging
作者:
C. J. Cogswell,
C. J. R. Sheppard,
期刊:
Journal of Microscopy
(WILEY Available online 1992)
卷期:
Volume 165,
issue 1
页码: 81-101
ISSN:0022-2720
年代: 1992
DOI:10.1111/j.1365-2818.1992.tb04307.x
出版商: Blackwell Publishing Ltd
关键词: Nomarski;differential interference contrast (DIC);confocal microscopy;scanning laser microscopy;imaging theory;three‐dimensional imaging;phase contrast
数据来源: WILEY
摘要:
SUMMARYA technique for obtaining differential interference contrast (DIC) imaging using a confocal microscope system is examined and its features compared to those of existing confocal differential phase contrast (DPC) techniques as well as to conventional Nomarski DIC. A theoretical treatment of DIC imaging is presented, which takes into account the vignetting effect caused by the finite size of the lens pupils. This facilitates the making of quantitative measurements in DIC and allows the user to identify and select the most appropriate system parameters, such as the bias retardation and lateral shear of the Wollaston prism.
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