Growth of cells superinoculated onto irradiated and nonirradiated confluent monolayers
作者:
Hideo Matsuoka,
Hiroaki Ueo,
Keizo Sugimachi,
期刊:
Seminars in Surgical Oncology
(WILEY Available online 1990)
卷期:
Volume 6,
issue 1
页码: 48-52
ISSN:8756-0437
年代: 1990
DOI:10.1002/ssu.2980060110
出版商: John Wiley&Sons, Inc.
关键词: contact inhibition;cell mats;confluent monolayer;BALB/c 3T3 cell
数据来源: WILEY
摘要:
AbstractWe prepared confluent monolayers of normal BALB/c 3T3 cells and compared differences in the growth of four types of cells superinoculated onto these nonirradiated and irradiated monolayers. The test cells were normal BALB/c 3T3 A31 cells, a squamous cell carcinoma from a human esophageal cancer (KSE‐1), human fetal fibroblasts, and V‐79 cells from Chinese hamster lung fibroblasts. Cell growth was checked by counting the cell number, determining [3H]thymidine incorporation and assessing colony formation. We found that on nonirradiated monolayers, colony formation of human fetal fibroblasts and normal BALB/c 3T3 cells was completely inhibited. On irradiated cells, test cells did exhibit some growth. KSE‐1 cells, which had a low clonogenic efficiency on plastic surfaces, formed colonies on both irradiated and nonirradiated cells. On these monolayers, the clonogenic efficiency of V‐79 cells was also higher than that on plastic surfaces. We conclude that the nonirradiated monolayer of BALB/c 3T3 cells completely inhibits the growth of superinoculated normal BALB/c 3T3 and human fetal fibroblasts, while on the other hand, they facilitate the growth of neoplastic KSE‐1 and V‐79 cells by providing a surface for cell adherence and growth, without affecting the presence of normal cells in
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