Hexadecane-induced Skin Hyperplasia in the Hairless Rat: Time Course of Histological and Biochemical Events Related to the Synthesis of Polyamines and DNA
作者:
M. Bouclier,
B. Shroot,
G. Milano,
J. Ferracin,
A. Chatelus,
C.N. Hensby,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1988)
卷期:
Volume 1,
issue 1
页码: 24-29
ISSN:1660-5527
年代: 1988
DOI:10.1159/000210748
出版商: S. Karger AG
关键词: Hyperplasia;Ornithine decarboxylase activity;Polyamines;DNA
数据来源: Karger
摘要:
Several biochemical parameters including ornithine decarboxylase activity (ODC) and tissue polyamine levels were measured during the hexadecane-induced epidermal hyperplasia of hairless rat skin. Animals received three applications of 200 μ1 pure n-hexadecane on day 1. ODC activity and polyamine levels (putrescine, spermidine and spermine) in the epidermis were significantly increased and reached maximum elevations at 12 h after the start of n-hexadecane treatment with DNA synthesis peaking at 24 h. Histological studies confirmed a significant cellular edema at 24 h after the beginning of the treatment followed at 48 h by an epidermal hyperplasia which was maximum at 72 h. These data support the view that ODC activation, increased biosynthesis of polyamines and DNA are early events in epidermal cell hyperproliferation.
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