A very simple procedure for separation of carrier-free144Pr and continuous milking of144Pr from144Ce in a alumina column has been developed. Cerous cerium was oxidized and adsorbed as ceric iodate in the column, and the praseodymium was eluted out unadsorbed. Study of the β-decay half-life of144Pr proved that the separated product is of high radiochemical purity. The separation process is rapid and the yield is quantitative.