SummarySerum amylase phenotypes were determined for 1227 animals from three Austrian cattle breeds, Tyrolean Grey, Carinthian Blondvieh and Waldviertler Blondvieh. The phenotypes were determined for 450 offspring from families where both sire and dam were typed. The distribution of the 450 progeny phenotypes in which 30 of the 36 possible mating combinations occur is compatible with the hypothesis of three autosomal co‐dominant alleles.All six possible phenotypes were found in all three breed groups. Gene frequencies of amylase alleles as well as the probability of excluding wrong parentage were estimated for all three breed