Characterization of simian immunodeficiency virus‐specific T‐cell-mediated cytotoxic response of infected rhesus macaques
作者:
Benjamin Vowels,
M. Gershwin,
Murray Gardner,
Aftab Ahmed-Ansari,
Thomas McGraw,
期刊:
AIDS
(OVID Available online 1989)
卷期:
Volume 3,
issue 12
页码: 785-792
ISSN:0269-9370
年代: 1989
出版商: OVID
关键词: Cell-mediated immunity;cytotoxic T lymphocyte;simian immunodeficiency virus;AIDS
数据来源: OVID
摘要:
Four juvenile rhesus macaques were infected with simian immunodeficiency virus (SIV)MAC. Freshly isolated peripheral blood mononuclear cells (PBMC) from these SIVMAC-infected and from uninfected control macaques were assessed for cytotoxic T-lymphocyte (CTL) activity monthly for 7 consecutive months, beginning 2 months after infection. Target cells consisted of major histocompatibility complex (MHC) haploidentical parental PBMC which were stimulated with mitogen and then pulsed with heat-killed SIVMAC. CTL activity was demonstrated on all four infected animals. The effector cells are T cells which mediate cytotoxicity against SIVMAC-pulsed target cells in an MHC-restricted manner. Furthermore, the cytotoxicity is virus specific and predominantly, if not exclusively, mediated by CD8+T cells; it is also MHC class-I restricted. Incubation of target cells with leupeptin prior to the cytotoxic assay inhibited target cell generation, suggesting that viral antigens are processed via an endocytic pathway.
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