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Amperometric Sensor and Immobilized Enzyme Electrode for the Determination of Enzymatic Activities

 

作者: L.J. Blum,   C. Bertrand,   P.R. Coulet,  

 

期刊: Analytical Letters  （Taylor Available online 1983）
卷期: Volume 16, issue 7  

页码: 525-540

 

ISSN:0003-2719

 

年代: 1983

 

DOI:10.1080/00032718308065189

 

出版商: Taylor & Francis Group

 

关键词: Enzyme electrodes;Electrochemistry;Enzyme activity;Amperometry;Hydrogen peroxide;Glucose monitoring;Immobilized enzyme

 

数据来源: Taylor

 

摘要:

A sensitive method for the rapid determination of activities of soluble or immobilized enzymes, based on the electrochemical detection of hydrogen peroxide is described. Kinetic studies (Vmax and KMdeterminations) can be performed for all H2O2generating enzymes (i.e. most of the oxidases) using an amperometric probe with a platinum anode at a fixed potential.

 

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