AbstractSteroid hormone receptors were studied in 45 patients with primary, recurrent, or metastatic ovarian cancer in cryostat‐frozen sections and imprint preparations. The ligands, 17 B‐estradiol‐6‐carboxymethyloxine‐bovine serum albumin fluorescein isothiocyanate (FITC‐BSA estradiol) and hydroxypro‐gesteronehemisuccinate bovine serum albumin tetramethylrho‐damine isothiocyanate (TMRITC‐BSA progesterone) were used in the fluorescent cytochemical method. Results were compared with standard dextran‐coated charcoal (DCC) biochemical assay. An overall significant correlation between biochemical values and cytochemical results was found. However, the imprint results were more sensitive and more specific than the frozen section results. A statistically significant difference (P<0.05) was observed between touch preparation material and frozen section specimens by the