Identification of hantavirus serotypes by testing of post‐infection sera in immunofluorescence and enzyme‐linked immunosorbent assays
作者:
J. Groen,
H. G. M. Jordans,
J. P. G. Clement,
E. J. M. Rooijakkers,
F. G. C. M. UytdeHaag,
J. Dalrymple,
G. Van Der Groen,
A. D. M. E. Osterhaus,
期刊:
Journal of Medical Virology
(WILEY Available online 1991)
卷期:
Volume 33,
issue 1
页码: 26-32
ISSN:0146-6615
年代: 1991
DOI:10.1002/jmv.1890330106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Hantavirus;Hällnäs virus;serological systems;serotype analysis
数据来源: WILEY
摘要:
AbstractSerum samples were collected from 27 individuals who had been infected with a member of the genus Hantavirus in the Netherlands or Belgium during the last 15 years. These samples were tested in an immunofluorescence assay (IFA) and two enzyme‐linked immunosorbent assay (ELISA) systems, using different virus strains that represented each of the four recently proposed serotypes of this genus. The serum samples from 11 individuals who had been infected through contacts with laboratory rats showed the highest reactivities with Hantaan virus (serotype I) and SR‐11 (serotype 11) in the IFA and ELISA systems. The samples of 16 individuals who had probably been infected through contacts with wild rodents showed the highest reactivities with Hällnäs virus (serotype III) in the IFA. All except two of these also showed the highest reactivity with Hällnäs virus in the two different ELISA
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